Affiliated to University of Kota, Kota
Recognized by Govt. of Rajasthan

9929144076 ,9468641732,9929144076

Value Added Certificate Cum Training Programmes

Eligibility: 12th pass or Graduate

For Registration & further information please contact: Modi Educational Complex, Dadabari Ext. , Kota-324009

Tel.- +91-0744-2505421, Tollfree- 1800-123-7701.

Separate hostel facilities for girls & boys. Hostel fee starts from 6500/- included breakfast, lunch & dinner (A.C. & Cooler rooms)

Advanced Biology

Module III : Molecular Biology and Immunological Techniques (Duration - 15 days /30 days/45 days )

Working in Molecular Biology Laboratory. Preparation of Reagents, Stock Solutions & Methods of Labeling and Storage.

General and Safety Instructions.

Extraction of Genomic DNA from Plants material.

To Perform Isolation of genomic DNA using CTAB method.

Purification of extracted plant genomic DNA material.

Electrophoresis of Genomic DNA.

Alginate for cell immobilization

Extraction of Plasmid DNA from E. coli

Purification of the extracted Plasmid DNA.

Electrophoresis of purified Plasmid DNA.

Perform the technique of sucrose centrifugation to isolate sub cellular fraction and identify it by using Janus green.

Introduction of SDS page electrophoresis.

Perform an experiment to isolate the Genetic material from the given animal tissue (Goat liver).

To perform & analyze the technique of Ouchterlony Double Diffusion.

To test the antigen sample by sandwich “Dot Elisa” method

Restriction Digestion of DNA by Restriction Endonucleases & Separation of Digestion Fragments by Electrophoresis.

To perform & analyze the technique of Rocket immune electrophoresis

To perform & analyze the technique of Radial immune diffusion.